Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target
Reexamination Certificate
2006-10-10
2006-10-10
Ceperley, Mary E. (Department: 1641)
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Cell membrane or cell surface is target
C435S173600, C435S029000, C436S172000, C436S182000, C436S800000, C436S805000
Reexamination Certificate
active
07118899
ABSTRACT:
Methods and compositions are provided for detecting changes in membrane potential in membranes biological systems. In one aspect, the method comprises;a) providing a living cell with a first reagent comprising a charged hydrophobic molecule which is typically a fluorescence resonance energy transfer (FRET) acceptor or donor, or is a quencher and is capable of redistributing within the membrane of a biological membrane in response to changes in the potential across the membrane;b) providing the cell with a second reagent that can label the first face or the second face of a biological membrane within the cell;c) detecting light emission from the first reagent or the second reagent.One aspect of this method involves monitoring membrane potential changes in subcellular organelle membranes in a living cells.Another aspect of the invention is the use of certain embodiments of the method for the screening of test chemicals for activity to modulate the activity of a target ion channel.Another aspect of the present invention is a transgenic organism comprising a first reagent that comprises a charged hydrophobic fluorescent molecule, and a second reagent comprising a bioluminescent or naturally fluorescent protein.
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Gonzalez, III Jesus E.
Tsien Roger Y.
Ceperley Mary E.
The Regents of the University of California
Townsend and Townsend / and Crew LLP
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