Detection of target nucleic acid molecules using synthesis-defic

Details Detection of target nucleic acid molecules using synthesis-defic Detection of target nucleic acid molecules using synthesis-defic

1995-06-06

1997-11-25

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435183, 436 94, 536 243, 536 2433, 935 78, C12Q 168, C12P 1934, C07H 2104, G01N 3348

Patent

active

056911424

ABSTRACT:
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. In this novel detection method, the signal indicative of the presence of the target nucleic acid sequence is amplified through cleavage of detection molecule(s) which comprise hairpin structures.

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