Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-03-22
2005-03-22
Graser, Jennifer E. (Department: 1645)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S975000, C536S023700, C536S024320, C536S024330
Reexamination Certificate
active
06869767
ABSTRACT:
The present invention relates to oligonucleotide primers, and methods of diagnosis using the primers, wherein particular primer pairs prime the universal amplification of an amplicon for specificStreptococcus pneumoniaefrom all 90 known serotypes. The amplicon includes at least a portion of the psaA gene ofSteptococcus pneumoniae. In the methods, amplification of a biological sample using the primer pairs of the invention provide an amplicon only if the sample contains a serotype ofStreptococcus pneumoniae. The invention further relates to an isolated nucleic acid including a nucleic acid amplicon obtained using a pair of oligonucleotide primers of the invention. In still a further aspect, the invention discloses a polypeptide including an amino acid sequence encoded by a nucleic acid amplicon obtained using oligonucleotide primers. Additionally, methods of stimulating an immune response against a serotype ofStreptococcus pneumoniaein a mammal include either administering a polypeptide encoded by a nucleic acid amplicon obtained using the pair of oligonucleotide primers of the invention, or administering a nucleic acid containing a nucleic acid amplicon obtained using the pair of oligonucleotide primers of the invention.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5422427 (1995-06-01), Russell et al.
patent: 5545522 (1996-08-01), Van Gelder et al.
patent: 5578467 (1996-11-01), Schuster et al.
patent: 5624833 (1997-04-01), Gelfand et al.
patent: 5854416 (1998-12-01), Sampson et al.
patent: 0 622 081 (1994-11-01), None
patent: 9818930 (1998-05-01), None
Sampson. Submitted (Apr. 1996). Gencore Accession No. U3509.*
Centers for Disease Control and Prevention, “Prevention of pneumococcal disease: Recommendation of the Advisory Committee on Immunization Practices,” (ACIP), 1997 Morbid. Mortal. Weekly Rep. (RR-8) 46:1-24 (Abstract).
Crook et al., “Immunoreactivity of five monoclonal antibodies against the 37-kilodalton common cell wall protein (PsaA) ofStreptococcus pneumoniae,” Clin. Diagn. Lab. Immunol.5:205-210 (1998).
Henrichsen, “Six newly recognized types ofStreptococcus pneumoniae,” J. Clin. Microbiol.33:2759-2762 (1995).
Kalin et al., “Diagnosis of pneumococcal pneumonia: a comparison between microscopic examination of expectorate, antigen detection, and cultural procedures,”Scand. J. Infect. Dis.15:247-255 (1983).
Novak, R. et al., “Penicillin tolerance genes ofStreptococcus pneumoniae: the ABC type maganese permease complex Psa,”Mol. Microbiology29(5):1285-1296 (Sep. 1998).
Obaro et al., “Carriage of pneumococci after pneumococcal vaccination,”Lancet348:271-272 (1996).
Russell et al, “Monoclonal antibody recognizing a species-specific protein fromStreptococcus pneumoniae, ” J. Clin. Microbiol.28:2192-2195 (1990).
Sampson et al., “Limited diversity ofStreptococcus pneumoniae psaAamong pneumococcal vaccine serotypes,”Infection and Immunity65(5):1967-1971 (May 1997).
Sampson, J.S. et al., “Cloning and nucleotide sequence analysis of psaA, theStreptococcus pneumoniaegene encoding a 37-kilodalton protein homologous to previously reportedStreptococcussp. adhesins,”Infection and Immunity62(1): 319-324 (Jan. 1, 1994).
Talkington et al., “Protection to mice against fatal pneumococcal challenge by immunization with pneumococcal surface adhesin A (PsaA),”Microbial Pathogenesis21(1):17-22 (Jul. 1996).
Tharpe et al., “Comparison of a pneumoccocal common protein (PsaA) antibody ELISA and a PsaA immune complex ELISA for detection of pneumococcal serum antibody,”Pathobiology66:77-83 (1998).
Tharpe et al., “Purification and seroreactivity of pneumococcal surface adhesin A (PsaA),”Clin. Diagn. Lab. Immunol.3(2):227-229 (Mar. 1996).
Tharpe et al, “The utility of a recombinant protein in an enzyme immunoassay for antibodies againstStreptococcus pneumoniae,” Abstr. V-2, p. 617, American Society for Microbiology, Washington, D.C. (1994).
Toikka et al., “Pneumolysin PCR-based diagnosis of invasive pneumococcal infection in children,”J. Clin. Microbiol.37:633-637 (1998).
Ades Edwin
Carlone George M.
Morrison Katherine E.
Sampson Jacqueline S.
Whitmon Jennifer Crook
Graser Jennifer E.
Needle & Rosenberg, PC.
The United States of America as represented by the Secretary of
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