Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-11-27
1988-09-13
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
935 78, 436800, 436805, C12Q 168
Patent
active
047709928
ABSTRACT:
A method for detection of DNA sequences. In the method, chromatin (comprising protein and DNA) is contacted with a cross-linking agent for the protein of the chromatin to provide a substantially rigid chromatin particle. The DNA of the chromatin particle is then subjected to treatment to cause a separation of the individual DNA strands into single stranded DNA. Preselected sequences of the single stranded DNA are then contacted with a complementary polynucleotide probe specific for the DNA sequence of interest. The polynucleotide probe is marked with a fluorescent label thereby labelling a target sequence of DNA in the chromatin particles. The fluorescently tagged DNA sequences are then detected by subjecting the polynucleotide probe to a suitable light source and detecting the light emitted by the fluorescent label so as to identify the preselected DNA sequence.
REFERENCES:
patent: 4585736 (1986-04-01), Dolbeare et al.
Bernheim et al., Proc. Natl. Acad. Sci (USA) 80, pp. 7571-7575, (1983).
Trask et al., Science 230, pp. 1401-1403, Dec., 1985.
Trask Barbara J.
Van den Engh Gerrit J.
Benson Robert
Hallenbeck Robert M.
Warden Robert J.
LandOfFree
Detection of specific DNA sequences by flow cytometry does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection of specific DNA sequences by flow cytometry, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of specific DNA sequences by flow cytometry will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-806687