Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1984-10-17
1988-06-21
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 7, 435 29, 436514, 436515, 436519, 436527, 436535, 436541, 436820, G01N 33543, G01N 33558
Patent
active
047525622
ABSTRACT:
Disclosed is a method for detection for serum antibody and/or microbial surface antigen by radial partition immunoassay. The method of this invention is applicable to (a) the evaluation of a clinical specimen for identification of a microorganism; (b) antimicrobial sensitivity assays for determination of an efficacious antibiotic for use against a specific microorganism; (c) a semi-quantitative determination of viral surface antigen; and, (d) a quantitative method for the determination of the presence of serum antibodies to microbial antigens. In each of the foregoing applications, the analyte of interest can be immobilized within a porous matrix (solid phase) by simple pipetting of the sample onto the prepared matrix. Appropriate reagents are subsequently applied to the matrix to effect immunochemical interaction of a labeled binding material to the surface antigen (or antibody) of the analyte of interest. The portion of the matrix within which such interaction takes place is termed the "reaction zone". After a brief incubation period, a suitable wash solution is then applied to the reaction zone of the matrix so as to effect radial partitioning, entirely within the matrix, of bound and unbound labeled binding material. The amount of signal generated by the bound label remaining in the reaction zone is then monitored visually or by an appropriate measuring device.
REFERENCES:
patent: 4366241 (1982-12-01), Tom et al.
patent: 4517288 (1985-05-01), Giegel et al.
Brotherton Mary
Giegel Joseph L.
Sheiman Mark I.
Baxter Travenol Laboratories Inc.
Benson Robert
Fentress Susan B.
Flattery P. C.
Hartenberger R. E.
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