Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-09-07
1998-09-15
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 72, 435 732, 435174, 435243, 4352528, G01N 33569, G01N 3353, C12N 120
Patent
active
058076948
ABSTRACT:
A method of testing for the presence of a pathogenic microorganism, notably Salmonella enteritidis, in a foodstuff, e.g. the contents of an egg, or other materials. In a particularly preferred form for testing eggs, the method involves homogenizing a sample of the egg, incubating the egg contents to enrich any pathogen present, adding a detergent to release antigens from pathogen cell surfaces, heating the egg contents to gell them and then carrying out a dot-blot assay of the gelled egg material to detect the presence of the antigens. The method is particularly applicable to D.sub.1 serovars of Salmonella enteritidis. The antibody used in the method is preferably a novel IgG.sub.2.alpha. type immunoglobulin having a lambda light chain, designated as MAb 2F11, which can also be used for other methods of testing for Salmonella enteritidis and other D.sub.1 serovars, and for passive protection of poultry against infections by such pathogens.
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Economic Innovation and Technology Council, University of Manito
Johnson Nancy A.
Scheiner Toni R.
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