Detection of Ribosomal RNA using PNA probes

Details Detection of Ribosomal RNA using PNA probes Detection of Ribosomal RNA using PNA probes

1997-06-05

1999-11-16

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

514 44, 536 221, 536 231, 536 2431, 536 2532, C12Q 168, C07H 1900, C07H 2102, C07H 2104

Patent

active

059855630

ABSTRACT:
PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis.
Specific peptide nucleic acid (PNA) probes for detecting a sexual transmitted disease caused by Neisseria gonorrhoeae or Chlamydia trachomatis comprising N-(2-aminoethyl)glycine units in amide linkage with the glycine nitrogen connected to naturally occurring nucleobases or non-naturally occurring nucleobases by a methylene carbonyl linker and said probes capable of hybridizing to 16S or 23S rRNA or rDNA of Neisseria gonorrhoeae or Chlamydia trachomatis are described.
PNA is a very stable molecule with very high affinity for nucleic acid allowing a PNA probe to be shorter than conventional nucleic acid probes.

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