Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-30
2008-09-09
Monshipouri, Maryam (Department: 1656)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
07422857
ABSTRACT:
The present invention concerns an assay for the detection of polyketide synthetase (PKS) mRNA from the red tide dinoflagellateKarenia brevis. Another aspect of the invention concerns NASBA primers and a molecular beacon that enable detection of mRNA of one of the PKS genes. In another aspect, the present invention includes an internal control RNA (IC-RNA) that enables quantitative detection of the target as well as correction for inhibition of amplification. The assay of the invention provides a rapid and specific detection of the putative toxin-producing genes of the Florida Red Tide Organism. The assay of the invention is useful for determining the virulence of red tide blooms.
REFERENCES:
Millie, D.F. et al. “Using absorbance and fluorescence spectra too discriminate microalgal phylogentic grups and taxa”J. Phycol., 2001, 37:35.
Monshipouri Maryam
Saliwanchik Lloyd & Saliwanchik
University of South Florida
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