Detection of particular nucleotide sequences

Details Detection of particular nucleotide sequences Detection of particular nucleotide sequences

1985-02-15

1988-08-30

Warren, Charles F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435803, 935 78, 436811, C12Q 168

Patent

active

047677007

ABSTRACT:
A process for the detection of a particular polynucleotide sequence in a polynucleotide-containing test sample comprising forming an agarose gel having a depression therein, adding the test sample to said depression, putting agarose solution in the depression and permitting it to gel thereby to encase the test sample in agarose gel, denaturing the polynucleotide in the test sample within the agarose gel, under hybridization conditions contacting the test sample-containing gel with an oligonucleotide or polynucleotide probe having a sequence complementary with a sequence for which the sample is being tested, and determining the extent of hybridization.

REFERENCES:
patent: 4395486 (1986-07-01), Wilson et al.
Thein, S. L. et al., In: Human Genetic Diseases a Practical Approach (K. E. Davies, editor), IRL Press, Oxford, England, 1986, pp. 33-50.
Kennell, D. E., In Progress in Nucleic Acid Research and Molecular Biology (Davidson et al., ed.) vol. 11, 1971, Academic Press, N.Y., pp. 259-261.
Meinkoth, J. et al., Anal. Biochem., vol. 138, 1984, pp. 267-269.
Singer-Sam, J. et al., Proc. Natl. Acad. Sci. USA, vol. 80, 1983, pp. 802-806.
Conner, B. J. et al., Proc. Natl. Acad. Sci. USA, vol. 80, 1983, pp. 278-282.

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