Detection of nucleic acids using a hairpin forming oligonucleoti

Details Detection of nucleic acids using a hairpin forming oligonucleoti Detection of nucleic acids using a hairpin forming oligonucleoti

1993-03-22

1996-11-12

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2433, 536 243, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

055739067

ABSTRACT:
A process for the detection of a nucleic acid sequence in a homogeneous assay format using an energy transfer system is disclosed. This process utilizes a 5' labeled primer containing a selfcomplementary sequence in an amplification or extension process together with a subsequent detection step using a 3' labeled probe for the amplified or extended region. The labels will be close together in space after hybridizing the probe close to the short piece of double-stranded DNA resulting from backfolding of the selfcomplementary region of the primer which has been incorporated into the amplified or extended product. A new primer for use in this process is also disclosed.

REFERENCES:
Bannwarth et al. (1991) Helvetica Chimica Acta 74: 2000-2008.
Krupp et al. (1987) FEBS Lett. 212:271-275.
Uhlmann (1988) Gene 71:29-40.
Bej et al. (1991) Critical Reviews in Biochem & Mol. Biology 26(3/4):301-334.
Seela, et al., Nucleic Acids Research, 15:3113-3129 (1987) "Oligodeoxyribo-nucleotides containing 1,3-propanediol as nucleoside substitute".
Wu, et al., Genomics, 4:560-569 (1989) "The Ligation Amplification Reaction--Amplification of Specific DNA Seq. using Sequential Rounds of Template-Dep. Ligation".

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