Detection of nucleic acids in cells by thermophilic strand displ

Details Detection of nucleic acids in cells by thermophilic strand displ Detection of nucleic acids in cells by thermophilic strand displ

1997-01-10

1998-05-26

Campbell, Eggerton A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, C07H 2102, C07H 2104

Patent

active

057567026

ABSTRACT:
Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be perfonned on the same specimen.

REFERENCES:
patent: 5550025 (1996-08-01), Walker
patent: 5631147 (1997-05-01), Lohman et al.

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