Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-09-21
1997-05-20
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, C12P 1934, C12Q 168
Patent
active
056311478
ABSTRACT:
Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.
REFERENCES:
patent: 5523204 (1996-06-01), Singer et al.
G. T. Walker, et al. "Strand displacement amplification - an isothermal, in vitro DNA amplification technique" Nucl. Acids Res. 20, 1691-1696 (1992).
G. T. Walker, et al. "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system" Proc. Natl. Acad. Sci, USA 89, 392-396 (1992).
G. J. Nuovo, et al. "Detection of human papillomavirus DNA in formalin-fixed tissues by in situ hybridization after amplification by Polymerase Chain Reaction" Amer. J. Pathol.139, 847-854 (1991).
G. J. Nuovo, et al."An improved technique for the in situ detection of DNA after Polymerase Chain Reaction Amplification" Amer. J. Pathol. 139, 1239-1244 (1991).
G. J. Nuovo, et al. "In situ localization of PCR-amplified human and viral cDNAs" PCR Meth. Appl. pp. 117-123 (1992).
Cleve Mark V.
Lohman Kenton L.
Ostrerova Natalie V.
Reid Robert A.
Becton Dickinson and Company
Campbell Eggerton A.
Fugit Donna R.
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