Detection of nucleic acids in cells by strand displacement ampli

Details Detection of nucleic acids in cells by strand displacement ampli Detection of nucleic acids in cells by strand displacement ampli

1993-12-10

1996-06-04

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

935 77, 935 78, C12P 1034, C12Q 168

Patent

active

055232043

ABSTRACT:
Strand Displacement Amplification for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues. Excellent specimen morphology is preserved, and either DNA targets, or RNA targets, or both may be selectively amplified. In situ amplification by SDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.

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European Search Report, EP 94 30 9308; 31 Mar. 1995.

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