Detection of nucleic acids in cells by strand displacement ampli

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

935 77, 935 78, C12P 1034, C12Q 168

Patent

active

055232043

ABSTRACT:
Strand Displacement Amplification for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues. Excellent specimen morphology is preserved, and either DNA targets, or RNA targets, or both may be selectively amplified. In situ amplification by SDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4888278 (1989-12-01), Singer et al.
patent: 5168038 (1992-12-01), Tecott et al.
patent: 5270184 (1993-12-01), Walker et al.
Bagasra, O., et al., "Detection of Human Immunodeficiency Virus Type 1 Provirus in Mononuclear Cells by In Situ Polymerase Chain Reaction", 1992, N.E.J. Med., vol. 326, No. 21, pp. 1385-1391.
Embleton, M. J., et al., "In-cell PCR from mRNA: Amplifying and Linking the Rearranged Immunoglobulin Heavy and Light Chain V-genes Within Single Cells", 1992, Nucleic Acids Res., vol. 20, No. 15, pp. 3831-3837.
Embretson, J., et al., "Analysis of Human Immunodeficiency Virus-Infected Tissues by Amplification and in Situ Hybridization Reveals Latent and Permissive Infections at Single-cell Resolution", 1993, Proc. Natl. Acad. Sci. USA, vol. 90, pp. 357-361.
Haase, A. T., et al., "Amplification and Detection of Lentiviral DNA Inside Cells", 1990, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 4971-4975.
Lawrence, J. B., et al., "Subcellular Localization of Low-abundance Human Immunodeficiency Virus Nucleic Acid Sequences Visualized by Fluorescence In Situ Hybridization", 1990, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 5420-5424.
Lawrence, J. B., et al., "Highly Localized Tracks of Specific Transcripts Within Interphase Nuclei Visualized by In Situ Hybridization", 1989, Cell, vol. 57, pp. 493-502.
Nuovo, G. J. et al., "Rapid In Situ Detection of PCR-Amplified HIV-1 DNA", 1992, Diagnostic Molec. Pathol., vol. 1, No. 2, pp. 98-102.
Nuovo, G. J., et al., "Detection of Human Papillomavirus DNA in Formalin-fixed tissues by In Situ Hybridization After Amplification by Polymerase Chain Reaction", 1991, Am. J. Pathol., vol. 139, No. 4, pp. 847-854.
Nuovo, G. J. et al., "Rapid Communication: An Improved Technique for the In Situ Detection of DNA after Polymerase Chain Reaction Amplification", 1991, Am. J. Pathol., vol. 139, No. 6, pp. 1239-1244.
Patterson, B. K., et al., "Detection of HIV-1 DNA and Messenger RNA in Individual Cells by PCR-Driven In Situ Hybridization and Flow Cytometry", 1993, Science, vol. 260, pp. 976-979.
Saiki, R. K., et al., "Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia", 1985, Science, vol. 230, pp. 1350-1354.
Walker, G. T., et al., "Isothermal In Vitro Amplification of DNA by a Restriction Enzyme/DNA Polymerase System", 1992, Proc. Natl. Acad. Sci. USA, vol. 89, pp. 392-396.
Walker, G. T. et al., "Strand Displacement Amplification--An Isothermal, In Vitro DNA Amplification Technique", 1992, Oxford Univ. Press, vol. 20, No. 7, pp. 1691-1696.
Bagasra, et al., "Human Immunodeficiency Virus Type 1 Provirus Is Demonstrated in Peripheral Blood Monocytes In Vivo: A Study Utilizing and In Situ Polymerase Cahin Reaction", 1993, AIDS Research and Human Retroviruses, 9:69-75.
Lohman, et al., "Detection of Intracellular HIV-1 Proviral DNA Sequences by Strand Displacement Amplification and In Situ Hybridization", 1993, Molecular Biol. of the Cell, vol. 4 Suppl., No. 656.
Walker, "Empirical Aspects of Strand Displacement Amplification", 1993, PCR Methods and Applications, 3:1-6.
European Search Report, EP 94 30 9308; 31 Mar. 1995.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Detection of nucleic acids in cells by strand displacement ampli does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of nucleic acids in cells by strand displacement ampli, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of nucleic acids in cells by strand displacement ampli will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-382581

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.