Detection of nucleic acids by formation of template-dependent pr

Details Detection of nucleic acids by formation of template-dependent pr Detection of nucleic acids by formation of template-dependent pr

1995-06-07

1999-03-16

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C12N 1500

Patent

active

058828672

ABSTRACT:
A method is disclosed for detecting a target polynucleotide sequence. The method comprises incubating an oligonucleotide with the target polynucleotide sequence and a nucleotide polymerase under isothermal conditions wherein at least one nucleotide is added to the 3'-terminus of the oligonucleotide to provide an extended oligonucleotide having the additional nucleotides. The presence of extended oligonucleotide is detected as an indication of the presence of the target polynucleotide sequence. The method has particular application to the detection of DNA.

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