Detection of nucleic acid heteroduplex molecules by denaturing h

Details Detection of nucleic acid heteroduplex molecules by denaturing h Detection of nucleic acid heteroduplex molecules by denaturing h

1995-08-08

1998-08-18

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 221, 536 231, 536 243, 435 6, 435 912, 204450, 204456, 2041828, C12N 1510, C07H 2102, C12Q 168, C12P 1934

Patent

active

057959765

ABSTRACT:
The present invention describes a method for separating heteroduplex and homoduplex DNA molecules in a mixture. In the method, such a mixture is applied to a stationary reverse phase support. The heteroduplex and homoduplex molecules are eluted with a mobile phase containing an ion-pairing reagent and an organic solvent. The eluting is carried out under conditions effective to at least partially denature the heteroduplexes (e.g., thermal or chemical denaturing) resulting in the separation of the heteroduplexes from the homoduplexes. The method has many applications including, but not limited to, comparative nucleic acid sequencing, linkage analysis, evolutionary studies, forensics, identification of disease-causing gene mutations, genetic marker development and diagnostics.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4965188 (1990-10-01), Mullis et al.
Agius, G., et al., "Variable Stringency Hybridization of Polymerase Chain Reaction Amplified HIV-1 DNA Fragments," J. Virol. Meth. 30:141-150 (1990).
Clay T.M., et al., "PCR-Fingerprinting for Selection of HLA Matched Unrelated Marrow Donors," The Lancet 337(8749):1049-1052 (1991).
Cotton, R.G.H., "Detection of Mutations in DNA," Current Opin. in Biotechnol. 3:24-30 (1992).
Cotton, R.G.H., "Current Methods in Mutation Detection," Mutation Res. 285:125-144 (1993).
Huber, C.G., et al., "Rapid and Accurate Sizing of DNA Fragments by Ion-Pair Chromatography on Alkylated nonporous Poly (styrene-divinylbenzene) Particles," Anal. Chem. 67:578-585 (1995).
Jin, L., et al., "Systematic Search for Polymorphisms in the Human Genome Using Denaturing High-Performance Liquid Chromatography (DHPLC)," Am. J. Human Genet. 57:A26 (Oct. 1995).
Kellogg, D.E., et al., "Quantitation of HIV-1 Proviral DNA Relative to Cellular DNA by the Polymerase Chain Reaction," Anal. Biochem. 189:202-208 (1990).
Oefner, P.J., et al., "High Resolution Liquid Chromatography of Fluorescent Dye-Labeled Nucleic Acids," Anal. Biochem. 223:39-46 (1994).
Oefner, P.J., et al., "High-Performance Liquid Chromatography for Routine Analysis of Hepatitis C Virus cDNA/PCR Products," Res. Reports 16:5 (1994).
Oefner, P., and Underhill, P.A., Comparative DNA Sequencing by Denaturing High-Performance Liquid Chromatography (DHPLC), Am. J. Human Genet. 57:A266 (Oct. 1995).
Underhill, P.A., et al., "Y-Chromosomal Variation and Recent Conquests in Deciphering Human Origins," Am. J. Human Genet. 57:A43 (Oct. 1995).
Zemans, R., et al., "Identification and Characterization of Pre-Columbian Human Y Chromosome Specific Allele," Am. J. Human Genet. 57:A174 (Oct. 1995).
Nagamine et al, "A PCR Artifact: Generation of heteroduplexes", Am. J. Hum. Genet. 45:337-339, 1989.
Prost et al, "The polymerase chain reaction and its applications", Meth. Mol. Cell. Biol. 1(2):45-51, Mar. 1989.
Keen et al, "Rapid detection of single base mismatches as heteroduplexes on hydrolink gels", Trends Genet. 7(1):5, Jan. 1991.
Hayward-Lester et al, "Rapid quantification of gene expression by competitive RT-PCR and lon-pair reversed phase HPLC", Biotechniques 20(2):250-257, Feb. 1996.

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