Detection of nucleic acid amplification

Details Detection of nucleic acid amplification Detection of nucleic acid amplification

1994-04-18

1996-08-20

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 5, 435 6, 536 243, C12P 1934, C12Q 170, C12Q 168, C07H 2104

Patent

active

055478611

ABSTRACT:
Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.

REFERENCES:
patent: 5126239 (1992-06-01), Livak et al.
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5348853 (1994-09-01), Wang et al.
Walker et al., Strand displacement amplification--an isothermal in vitro DNA amplification technique, NAR 20: 1691-1696, 1992*.
WO9201812--Uhlen et al. Competitive PCR for quantitations of DNA, pp. 1-19, pub. Feb. 6, 1992*.
G. T. Walker, et al. "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system" Proc. Natl. Acad. Sci. USA 89, 392-396 (1992).
C. P. H. Vary "Triple-Helical Capture Assay for Quantirfication of Polymerase Chain Reaction Products" Clin. Chem. 38, 687-694 (1992).
J. Wahlberg, et al. "General colorimetric method for DNA diagnostics allowing direct solid-phase genomic sequencing of the positive samples" Proc. Natl. Acad. Sci. USA 87, 6569-6573 (1990).
D. J. Kemp, et al. "Colorimetric detection of specific DNA segments amplified by polymerase chain reactions" Proc. Natl. Acad. Sci. USA 86, 2423-2427 (1989).
F. F. Chehab, et al. "Detection of specific DNA sequences by fluorescence amplification: A color complimentation assay" Proc. Natl. Acad. Sci. USA 86, 9178-9182 (1989).
A. C. Syvanen, et al. "Quantification of polymerase chain reaction products by affinity-based hybrid collection" Nucl. Acids Res. 16, 11327-11338 (1988).
A. Chan, et al. "Quantification of Polymerase Chain Reaction Products in Agarose Gels with a Fluorescentn Europium Chelate as Label and Time-Resolved Fluorescence Spectroscopy" Anal. Chem. 65, 158-163 (1993).
C. R. Newton, et al. "The production of PCR products with 5' single-stranded tails using primers that incorporate novel phosphoramidite intermediates" Nucl. Acids. Res. 21, 1155-1162 (1993).
P. M. Holland, et al. "Detection of specific polymerase chain reaction product by utilizing the 5'-3' exonuclease activity of Thermus aquaticus DNA polymerase" Proc. Natl. Acad. Sci. USA 88, 7276-7280 (1991).
P. M. Holland, et al. "Detection of specific polymerase chain reaction product by utilizing the 5'-3' exonuclease activity of Thermus aquaticus DNA polymerase" Clin. Chem. 38, 462-463 (1992).

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