Detection of novel carbohydrates directly associated with chroni

Details Detection of novel carbohydrates directly associated with chroni Detection of novel carbohydrates directly associated with chroni

1994-05-27

1998-05-05

Soderquist, Arlen

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

436 93, 436127, 436129, 436131, 436161, 436162, 436169, 422 56, 422 61, G01N 3300, G01N 3090, G01N 3002, G01N 2177

Patent

active

057473463

ABSTRACT:
Chronic or long-term alcohol consumption is detected and monitored by determining the level of a newly-observed, alcohol-specific carbohydrate in body fluids (e.g. urine) of subjects by calorimetric reaction using qualitative and quantitative assay methods. The alcohol-specific carbohydrate have been identified as a novel ethyl glucuronide. Ethyl glucuronide is observed and detected in direct response to alcohol consumption in body fluids, and can be isolated and purified. Simple, economical, and reproducible assay methods, such as a spot assay and an ascending or thin layer chromatography assay, provide reliable, objective, and sensitive methods for detecting and monitoring a chronic alcoholic condition. Both the presence of the alcohol-specific ethyl glucuronide and a substantial increase in its levels are indicative of chronic alcoholism. Since the novel ethyl glucuronide is produced and appears as a direct response to chronic alcohol intake, the novel carbohydrate is considered to be a unique biomarker for the detection of alcoholism, with virtually no possibility of false positive results.

REFERENCES:
patent: 2524414 (1950-10-01), Wolfrom
patent: 2845439 (1958-07-01), Reiners
patent: 4246347 (1981-01-01), Neidleman et al.
patent: 4463098 (1984-07-01), Hoberman
patent: 4514563 (1985-04-01), Fujiyama et al.
patent: 4575551 (1986-03-01), Fujiyama et al.
patent: 4770996 (1988-09-01), Tabakoff
patent: 4837144 (1989-06-01), Sugiyama
I. Smith "Chromatographic Techniques" Interscience Publishers, Inc.: New York, 1958, pp. 164-177.
R.T. Williams "Detoxication Mechanisms" John Wiley & Sons Inc.: New York, 1959, pp. 46-55.
J. Kocourek et al. J. Chromatog. 1966, 24, 117-124.
T. Mizuno et al. Shizuoka Daigaku Nogakubu Kenyu Hokoku 1971, 29-37.
T. Kartnig et al. J. Chromatog. 1971, 61, 375-377.
T. Kartnig et al. Plant. Med. 1972, 21, 144-149.
H.A.G. Muller et al. Clin. Chim. Acta 1977, 78, 17-21.
N. Iizima et al. J. Chromatog. 1980, 193, 464-469.
T. Kawada et al. Agric. Biol. Chem. 1985, 49, 441-448.
E. Mentasti et al. J. Chromatog. 1985, 322, 177-189.
F. Caccamo et al. J. Chromatog. 1986, 362, 47-53.
J. G. Streeter Plant. Phsyiol. 1987, 85, 768-773.
B. Y Lee et al. LC-GC 1992, 10, 884-887.
K. Kawahara et al. Biochem. Biophys. Acta 1982, 712, 571-575.
R.K. Pallarkat et al. Alcohol. Clin. Exp. Res. 1985, 9, 28-30.
J.M Bobbitt "Thin Layer Chromatography" 1963, Reinhold Publishing Corporation: New York, 61-63.
H. Stibler et al. Alcohol. Clin. Exp. Res. 1986, 10, 61-64.
J. Iwamura et al. Chem. Abstr. 1986, 105, 108686d.
Y. Israel et al. Proc. Natl. Acad. Sci. 1986, 83, 7923-7927.
B.K. Tang et al. Biochem. Biophys. Res. Commun. 1986, 140, 924-927.
I.A. Kamil, et al. Biochem. J. 1953, 53, 129-136.
I.A. Kamil et al. Biochem. J. 1953, 53, 390-392.
H.R. Bollinger et al. "Thin-Layer Chromatography A Laboratory Handbook".
E. Stahl ed. 1965, Springer Verlag: New York, 14-25, 466-469 and 483-501.
T. Kozu Chem. Abstr. 1975, 83, 126992g.
Z. Zilic J. Chromatog. 1979, 164, 91-94.
D.M. Chambers et al. Gut 1981, 22,992-996.
K.O. Lewis et al. Brit. Med. J. 1981, 283, 1531-1532.
E.L. Storey et al. Lancet 1987, 1292-1293.
A Sieg et al. Gastroenterology 1987, 93, 261-265.
R.P. Risto et al. Alcohol. Clin. Exp. Res. 1987, 11, 525-527.
B. Tabakoff et al. Ne Eng. J. Med. 1988, 318, 134-139.
R. Klaus et al. Methods Enzym. 1988, 160, 159-175.
H. Stibler et al. Alcohol. 1988, 5, 393-398.
S.S. Narayan et al. Xenobiotica 1991, 21, 515-524.
B.Y. Lee et al. Chem. Abstr. 193, 118, 187131P.
T. Kozu, 1973, "Gas Chromatographic Analysis of Ethyl-.beta.-D-Glucuronide in Human Urine", Shinshu Igaku Kasahi, Shinsu Medical Journal, 21 (5)(6), 595-601.
P.I. Jaakonmaki et al., 1967, "The Characterization by Gas-Liquid Chromatography of Ethyl .beta.-D-Glucosiduronic Acid as a Metabolite of Ethanol in Rat and Man", Eur. J. Pharamacol., 1:63-70.
J.E. Manautou and G.P. Carlson, 1992, "Comparison of Pulmonary and Hepatic Glucuronidation and Sulphation of Ethanol in Rat and Rabbit in vitro", Xenobiotica, 22(11):1309-1319.
I.A. Kamil et al., 1952, "A New Aspect as Ethanol Metabolism: Isolation of Ethyl Glucuronide," Biochem. J., 51:22-23.

Affiliated with

Also associated with

Rating

Detection of novel carbohydrates directly associated with chroni does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of novel carbohydrates directly associated with chroni, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of novel carbohydrates directly associated with chroni will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-53562