Chemistry: analytical and immunological testing – Nitrogen containing
Reexamination Certificate
2005-04-12
2011-11-08
Bullock, In Suk (Department: 1772)
Chemistry: analytical and immunological testing
Nitrogen containing
C436S086000, C436S107000, C436S110000, C436S119000, C436S172000, C436S538000, C435S188000, C435S194000, C435S375000, C435S007500, C204S461000, C424S718000
Reexamination Certificate
active
08053243
ABSTRACT:
The present invention, in some aspects, relates to systems and methods for determining oxidized proteins, including nitrosylated proteins such as S-nitrosylated proteins. The systems and methods of the invention can be used in vitro (e.g., in cell or tissue culture) or in vivo. For instance, in some cases, the invention can be used to spatially determine the location and/or concentration of oxidized proteins within cells and/or tissues (e.g., through visual detection). In one set of embodiments, a nitrosylated or otherwise oxidized moiety on a protein may be reacted with a detection entity, which may be, for example, fluorescent, radioactive, electron-dense, able to bind to a signaling entity or a binding partner in order to produce a signal, etc. In some embodiments, other moieties on the protein may be altered or blocked before reaction of the protein with the detection entity. Such moieties on the protein may be, for instance, non-oxidized or non-nitrosylated moieties able to react with the detection entity. Also provided in certain aspects of the present invention are kits for determining oxidized proteins, which may include components such as detection entities, alkylating agents, blocking agents, reducing agents, signaling entities, binding partners, antibodies, instructions, and the like.
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Ckless Karina
Janssen Yvonne M.
Reynaert Niki
van der Vliet Albert
Bullock In Suk
The University of Vermont and State Agricultural College
White Dennis M
Wolf Greenfield & Sacks P.C.
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