Detection of neu p185 in cell lysates

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 71, 435 77, 435 792, 436 64, 436813, 5303888, 53038885, G01N 33574, G01N 3353

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active

056041071

ABSTRACT:
This invention relates to a substantially purified p100 which is a human neu related protein having a molecular weight in the range from about 97,000 daltons to about 115,000 daltons which corresponds substantially to the extracellular domain of the human neu gene product, said protein being detectable in a biological fluid.
In another embodiment this invention relates to assays for detecting this protein.
Finally, this invention also concerns monoclonal antibodies which are capable of binding to p100.

REFERENCES:
McKenzie, S. J., et al., Oncogene, 4,543-548, 1989.
Practice & Theory of Enzyme Immunoassays, P. Tijssen, Elsevier Science Pubs., Amsterdam, The Netherlands, 340-343, 1985.
Carney, et al., Journal of Tumor Marker Oncology, vol. 6, No. 2, pp. 53-72, 1991.
Gullick, et al., Int. J. Cancer, vol. 40, pp. 246-254, 1987.
Berger, et al, Cancer Res., vol. 48, pp. 1238-1243, Mar. 1, 1988.

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