Detection of Neisseria gonorrhoeae by amplification and detectio

Details Detection of Neisseria gonorrhoeae by amplification and detectio Detection of Neisseria gonorrhoeae by amplification and detectio

1997-11-04

1999-10-05

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 912, 536 221, 536 243, 536 253, 536 2532, C12Q 168, C12P 1934, C07H 2104, C07H 2100

Patent

active

059622733

ABSTRACT:
Methods for detecting the presence of Neisseria gonorrhoeae are described. These methods are based upon amplifying a portion of the Neisseria gonorrhoeae genome and detecting the presence of the amplified nucleic acid. Various sets of primers and detectors are disclosed. The disclosed primers and detectors can be used in Strand Displacement Amplification assays, thermal Strand Displacement Amplification Assays, and homogeneous, fluorescent real time thermal Strand Displacement Amplification assays to specifically detect the presence of Neisseria gonorrhoeae even in the presence of contaminating microorganisms and in the presence of human DNA.

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Donegan, James J., Isolation of a species-specific DNA probe for Neisseria gonorrhoeae using a novel technique particularly suitable for use with closely related species displaying high levels of DNA homology; Moll. Cell. Prob., 3, pp. 13-26 (1989).
Walker, G.T. et al., Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system; PNAS, 89, pp. 392-396 (1992).
Walker, G.T. et al., Strand displacement amplification--an isothermal, in vitro DNA amplification technique, Nucl. Acids Res., 20, pp. 1691-1696 (1992).
Database search report, Accesion No. U65994, 1996.

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