Detection of mycoplasma infection in cell cultures

Details Detection of mycoplasma infection in cell cultures Detection of mycoplasma infection in cell cultures

1981-07-16

1983-06-07

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 31, 435240, 435870, C12Q 104, C12Q 122, C12N 500, C12R 135

Patent

active

043871615

ABSTRACT:
Detection of mycoplasma infection in an animal cell culture is accomplished by growing animal cells from a culture in a growth medium in the presence of 6-methylpurine deoxyriboside and determining whether animal cells are killed.

REFERENCES:
patent: 3668075 (1972-06-01), Cekoric, Jr. et al.
Aaron Bendich et al., Ciba Foundation Symposium on the Chemistry and Biology of Purines; Little Brown & Co., pp. 18, 19, 75 and 76; 1957.
Masakazu Hatanka et al., Proc. Nat. Acad. Sci., USA, vol. 72, No. 4, pp. 1401-1405; 1975.
Gerard J. McGarrity et al., In Vitro, vol. 15, No. 2, pp. 73-81; 1979.
Gerard J. McGarrity et al., Experimental Cell Research, vol. 121, pp. 159-165; 1979.
S. Senesi et al., FEBS Letters, vol. 64, No. 2, pp. 353-357; 1976.
T. P. Zimmerman et al., Canadian Journal of Biochemistry, vol. 49, pp. 1050-1054; 1971.
Chemical Abstracts, vol. 95, 198115u; 1981.
In Vitro, vol. 12, pp. 643-648, (1977) by McGarrity.
In Vitro, vol. 9, pp. 17-18, (1973) by McGarrity.
Methods in Enzymology, vol. 58, pp. 18-29, (1978) by McGarrity.
Selective Killing of Leishmania Infected Mouse Macrophages by 6-Methypurine 2'-Deoxyriboside, by Carson & Chang, Life Sciences vol. 29, pp. 1617-1621.

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