Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-04-30
1998-09-22
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 912, 536 221, 536 2432, C12P 1934, C07H 2104
Patent
active
058112693
ABSTRACT:
Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of the Mycobacterium tuberculosis (M.tb) complex and a 16S rDNA target common to essentially all mycobacteria are described. In certain embodiments, the primers are optimized for efficient multiplex amplification in thermophilic SDA. The multiplex Strand Displacement Amplification methods of the invention are capable, in a single amplification reaction, of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of mycobacteria. Also disclosed are internal control sequences designed for coamplification with the two targets, allowing assessment of amplification efficiency and/or quantitation of the targets.
REFERENCES:
patent: 5422252 (1995-06-01), Walker et al.
patent: 5470723 (1995-11-01), Walker
Stackebrandt et al. Evidence of phylogenetic heterogeneity within the genus rhodococcus: revival of the genus gordon a (tsukamura), J. Gen. Appl. Microbiol., vol. 34, pp. 341-348, 1988.
Dean Cheryl H.
Dey Margaret S.
Howard Deborah R.
Nadeau James G.
Schram James L.
Becton Dickinson and Company
Fugit Donna R.
Horlick Kenneth R.
Tung Joyce
LandOfFree
Detection of mycobacteria by multiplex nucleic acid amplificatio does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection of mycobacteria by multiplex nucleic acid amplificatio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of mycobacteria by multiplex nucleic acid amplificatio will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1621141