Detection of mycobacteria by multiplex nucleic acid amplificatio

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 912, 536 221, 536 2432, C12P 1934, C07H 2104

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active

058112693

ABSTRACT:
Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of the Mycobacterium tuberculosis (M.tb) complex and a 16S rDNA target common to essentially all mycobacteria are described. In certain embodiments, the primers are optimized for efficient multiplex amplification in thermophilic SDA. The multiplex Strand Displacement Amplification methods of the invention are capable, in a single amplification reaction, of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of mycobacteria. Also disclosed are internal control sequences designed for coamplification with the two targets, allowing assessment of amplification efficiency and/or quantitation of the targets.

REFERENCES:
patent: 5422252 (1995-06-01), Walker et al.
patent: 5470723 (1995-11-01), Walker
Stackebrandt et al. Evidence of phylogenetic heterogeneity within the genus rhodococcus: revival of the genus gordon a (tsukamura), J. Gen. Appl. Microbiol., vol. 34, pp. 341-348, 1988.

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