Detection of mutations in nucleic acids

Details Detection of mutations in nucleic acids Detection of mutations in nucleic acids

1991-12-26

1993-06-08

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 232, 536 243, 536 2431, 935 77, 935 78, C12Q 168, C07H 1512, C07H 1700, C12N 1500

Patent

active

052178633

ABSTRACT:
A method of detecting point mutation in nucleic acids is described which comprises hybridizing a piece of control DNA or RNA without mutations with a piece of test DNA or RNA generally corresponding to the test DNA or RNA but possibly with mutations to produce a heteroduplex, treating the heteroduplex with hydroxylamine or osmium tetroxide and with piperidine, and subjecting the resulting material to separation treatment. Individual strands of both senses of control nucleic acid can be labelled in turn allowing detection of all possible mutations.

REFERENCES:
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Cotton et al., Proceedings National Academy of Sciences, USA, vol. 85, Jun. 1988, pp. 4397-4401.
Johnston et al., Biological Abstracts, Abstract No. 33128, vol. 81, 1986.
Lilley et al., Chemical Abstracts, Abstract No. 33961w, vol. 101, 1984, p. 125.
Cashmore et al., Chemical Abstracts, Abstract No. 84180t, vol. 75, 1971, p. 12.

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