Detection of mutation by resolvase cleavage

Details Detection of mutation by resolvase cleavage Detection of mutation by resolvase cleavage

1996-09-16

1997-12-16

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435911, 435912, 536 243, 536 2431, 536 2433, C12Q 168, C12Q 170, C12D 1134, C07H 2104

Patent

active

056984006

ABSTRACT:
Methods are disclosed for detecting one or more mutations in an isolated test nucleic acid by forming a heteroduplex with a homologous control DNA and contacting the heteroduplex with a resolvase capable of recognizing at least one single base pair mismatch within the heteroduplex. In preferred embodiments of the invention, the resolvase is bacteriophage T4 endonuclease VII.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5217863 (1993-06-01), Cotton et al.
patent: 5459039 (1995-10-01), Modrich et al.
Jensch et al, (1989), "Cruciform cutting endonucleases from Sacchromyces cerevisiae and phage T4 show conserved reaction with branched DNAs" EMBO J. 8:4325-4334.
Galindez et al, (1991), "Characterization of genetic variation and 3'-azido-3' deoxythymidine resistance mutations of human immunodeficiency virus by RNase A mismatch cleavage method" Proc. Natl. Acad. Sci. 88:4280-4284.
Forrest et al, (1992), "Use of chemical cleavage of mismatch method for prenatal diagnosis of alpha-1-antitrypsin deficiency" Prenatal Diagnosis 12:133-137.
Winter et al, (1985), "A method to detect and characterize point mutations in transcribed genes: Amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells" Proc. Natl. Acad. Sci. 82:7575-7579.
Beck et al, (Mar. 1993), "A denaturing gradient gel electrophoresis assay for sensitive detection of p53 mutations" Hum Genet 91:25-30.
Telenti et al, (Mar. 1993), "Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis", Lancet 341:647-650.
Bhattacharyya et al.; Model for the Interaction of DNA Junctions and Resolving Enzymes; 1991 Academic Press Limited; 20:1191-17; 1991.
Youil, et al.; Poster Symposium -Session 40; The American Society of Human Genetics; 53:1257; 1993.
Cotton; Detection of Mutations in DNA; Current Biology Ltd; 3:24-30; 1992.
Cotton; Current Methods of Mutation Detection; Mutation Research; 285:125-144; 1993.
Dahl et al.; Pyruvate Dehydrogenase Deficiency Caused by Deletion of a 7-BP Repeat Sequence in the Ela Gene; Am. J. Hum. Genet.; 47:286-293; 1990.
DiLella et al.; Screening for Phenylketonuria Mutations by DNA Amplification with the Polymerase Chain Reaction; The Lancet; 497-499; Mar. 5, 1988.
Kemper et al.; Resolution of Holliday Structures by Endonuclease VII as Observed in Interactions with Cruciform DNA; Cold Spring Harbor Symp. Quant. Biol. 49:815-825, 1984.
Kleff et al.; Initiation of Heteroduplex-Loop Repair by T4-Encoded Endonuclease VII in Vitro; The EMBO Journal; 7:1527-1535; 1988.
Kosak et al.; Large-Scale Preparation of T4 Endonuclease VII from Over-Expressing Bacteria; Eur. J. Brochem.; 194:779-784; 1990.
Lilley et al.; Cruciform-Resolvase Interactions in Supercoiled DNA; Cell; 36:413-422; 1984.
Lin et al.; Geographical Clusters of Dengue Virus Type 2 Isolates Based on Analysis of Infected Cell RNA by The Chemical Cleavage at Mismatch Method; Journal of Virological Methods; 40:205-218; 1992.
Lu et al.; Detection of Single DNA Base Mutations with Mismatch Repair Enzymes; Genomics; 14:249-255; 1992.
Mizuuchi et al.; T4 Endonuclease VII Cleaves Holliday Structures; Cell; 29:357-365; 1982.
Mueller et al.; T4 Endonuclease VII Cleaves the Crossover Strands of Holliday Junction Analogs; Proc. Natl. Acad. Sci. USA; 85:9441-9445; 1988.
Muller et al.; Enzymatic Formation and Resolution of Holliday Junctions In Vitro; Cell; 60:329-336; 1990.
Parsons et al.; Resolution of Model Holliday Junctions by Yeast Endonuclease is Dependent Upon Homologous DNA Sequences; Cell; 52:621-629; 1988.
Parsons et al.; Interaction of a Four-Way Junction in DNA with T4 Endonuclease VII*; The Journal of Biological Chemistry; 265:9285-9289; 1990.
Pottmeyer et al.; T4 Endonuclease VII Resolves Cruciform DNA with Nick and Counter-Nick and Its Activity is Directed by Local Nucleotide Sequence; J. Mol. Biol.; 223:607-615; 1992.
Shenk et al.; Biochemical Method for Mapping Mutational Alterations in DNA with S1 Nuclease: The Location of Deletions and Temperature-Sensitive Mutations in Simian Virus 40*; Proc. Nat. Acad. Sci.; 72:989-993; 1975.
Smooker et al.; Identification and In Vitro Expression of Mutations Causing Dihydropteridine Reductase Deficiency; Biochemistry; 32:6443-6449; 1993.
Solaro et al.; Endonuclease VII of Phage T4 Triggers Mismatch Correction In Vitro; J. Mol. Biol., 230:868-877; 1993.
West; Enzymes and Molecular Mechanisms of Genetic Recombination; Annu. Rev. Biochem.; 61:603-40; 1992.

Affiliated with

Also associated with

Rating

Detection of mutation by resolvase cleavage does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of mutation by resolvase cleavage, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of mutation by resolvase cleavage will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-204639