Detection of morphine and its analogues using enzymatic hydrolys

Details Detection of morphine and its analogues using enzymatic hydrolys Detection of morphine and its analogues using enzymatic hydrolys

1982-06-03

1984-09-25

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435264, 435268, 435803, C12Q 134

Patent

active

044736401

ABSTRACT:
The invention relates to a method for hydrolyzing drug-glucuronic acid conjugates present in mammalian body fluids, the conjugates being derived from a narcotic analgesic, antagonist, or agonist-antagonist whose metabolism includes conjugation with glucuronic acid. The method comprises incubating the body fluid sample at from about 60.degree. to about 70.degree. C., for at least about 1 hour, with .beta.-glucuronidase derived from Patella vulgata, and substantially increases the sensitivity of chromatographic techniques for the detection of morphine and its analogues.

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