Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-05-29
2007-05-29
Lu, Frank (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S455000, C536S023100, C536S024300
Reexamination Certificate
active
09953496
ABSTRACT:
Methods and compositions for detecting molecular interactions, particularly protein-protein interactions, are provided. The invention allows detection of such interactions in living cells or in vitro. Detection of molecular interactions in living cells is not limited to the nuclear compartment, but can be accomplished in the cytoplasm, cell surface, organelles, or between these entities. In one embodiment, the method utilizes novel compositions comprising fusion proteins between the molecules of interest and two or more inactive, weakly-complementing β-galactosidase mutants. Association between the molecules of interest brings the complementing β-galactosidase mutants into proximity so that complementation occurs and active β-galactosidase is produced. The active β-galactosidase may be detected by methods well-known in the art. Among the uses of the invention are the study of protein-protein interactions, functional genomics, agonist and antagonist screening and drug discovery.
REFERENCES:
patent: 4708929 (1987-11-01), Henderson
patent: 5223393 (1993-06-01), Khanna et al.
patent: 5223402 (1993-06-01), Abbas et al.
patent: 5362625 (1994-11-01), Krevolin et al.
patent: 5434052 (1995-07-01), Khanna
patent: 5503977 (1996-04-01), Johnsson et al.
patent: 5585245 (1996-12-01), Johnsson et al.
patent: 5958705 (1999-09-01), Staunton et al.
patent: 6312939 (2001-11-01), Roberts et al.
patent: 6342345 (2002-01-01), Blau et al.
patent: 2196496 (1998-07-01), None
patent: 0 601 889 (1994-06-01), None
patent: WO 95/07463 (1995-03-01), None
patent: WO 96/23810 (1996-08-01), None
patent: WO 96/30540 (1996-10-01), None
patent: WO 96/41166 (1996-12-01), None
patent: WO 98/34120 (1998-08-01), None
patent: WO 98/44350 (1998-10-01), None
Kraft et al., Parallel inhibition of active force and relaxed fiber stiffness by caldesmon fragments at physiological ionic strength and temperature conditions. Biophysical Journal, 68, 2404-2418, 1995.
Gayatri et al., Complementation of souble phosphofructokinase activity in yeast mutants. Eur. J. Biochem., 149-155, 1991.
Adams et al., “Fluorescence ratio imaging of cyclic AMP in single cells” (1991)Nature 349: 694-697.
Alberti et al., “A single laser method for subtraction of cell autofluorescence in flow cytometry” (1987)Cytometry 8:114-119.
Bai et al., “Gene identification using the yeast two-hybrid system” (1996)Meth. Enzymol. 273:331-347.
Beckwith, J.R., “A deletion analysis of theLacoperator region inEscherichia coli” (1964)J. Mol. Biol. 8:427-430.
Beerli et al., “Epidermal growth factor-related peptides activate distinct subsets of ErbB receptors and differ in their biological activities” (1996)J. Biol. Chem. 271:6071-6076.
Belshaw et al., “Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimerization of proteins” (1996)Proc. Natl. Acad. Sci. USA 93: 4604-4607.
Benezra et al., “The protein Id: A negative regulator of helix-loop-helix DNA binding proteins” (1990)Cell 61:49-59.
Bronstein et al. “1,2-dioxetanes: Novel chemiluminescent enzyme substrates. Applications to immunoassays” (1989)J. Biolumin. Chemilumin. 4:99-111.
Brown et al., “A mammalian protein targeted by G1-arresting rapamycin-receptor complex” (1994)Nature 369: 756-758.
Capecchi, M.R., “Altering the genome by homologous recombination” (1989)Science 244: 1288-1292.
Chen, Z-F., “Twistis required in head mesenchyme for cranial neural tube morphogenesis” (1995)Genes Devel. 9:686-699.
Chen et al., “I-mf, a novel myogenic repressor, interacts with members of the MyoD family” (1996)Cell 86:731-741.
Chen et al., “Identification of an 11-kDa FKBP12-rapamycin-binding domain within the 289-kDaFKBP12-rapamycin-associated protein and characterization of a critical serine residue” (1995)Proc. Natl. Acad. Sci. USA 92:4947-4951.
Choi et al., “Structure of the FKBP12-rapamycin complex interacting with the binding domain of human FRAP” (1996)Science 273:239-242.
Cossu et al. “How is myogenesis initiated in the embryo?” (1996)Trends Genet. 12:218-223.
Dhawan et al., “Systemic delivery of human growth hormone by injection of genetically engineered myoblasts” (1991)Science 254:1509-1512.
Dunn, I.S. and Jennings, P.A. “Protein modification from mutational analysis of an autologous peptide fragment” (1990)Protein Engineering 4(2):205-213.
Felder et al. “Kinetics of binding, endocytosis, and recycling of EGF receptor mutants” (1992)J. Cell. Biol. 117:203-212.
Fields et al., “A novel genetic system to detect protein-protein interactions”(1989)Nature 340:245-246.
Fiering et al. “Improved FACS-Gal: Flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs” (1991)Cytometry12:291-301.
Fiering et al., “Targeted delection of 5′HS2 of the murine β-globin LCR reveals that it is not essential for proper regulation of the β-globin locus” (1995)Genes Dev. 9:2203-2213.
Füchtbauer, E-M., “Expression of M-twist during postimplantation development of the mouse” (1995)Dev. Dyn. 204:316-322.
Gadella, Jr. et al., “Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation” (1995)J. Cell Biol. 129:1543-1558.
Hebrok et al., “M-twistis an inhibitor of muscle differentiation” (1994)Dev. Biol. 165:537-544. (1994).
Hinrichs et al., “Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance” (1994)Science 264:418-420.
Ho, S. N. et al., “Dimeric ligands define a role for transcriptional activation domains in reinitiation” (1996)Nature 382:822-826.
Hu et al., “HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulator factors” (1992)Mol. Cell. Biol. 12:1031-1042.
Hughes et al., “Migration of myoblasts across basal lamina during skeletal muscle development” (1990)Nature 345:350-353.
Jackson et al. “Restoration of enzymic activity by complementationin vitrobetween mutant α subunits of tryptophan synthetase and between mutant subunits and fragments of the α subunit” (1969)J. Biol. Chem. 244:4539-4546.
Jacobson et al., “Three-dimensional structure of β-galactosidase fromE. coli” (1994)Nature 369:761-766.
Johnsson Nils and Alexander Varshavsky “Split ubiquitin as a sensor of protein interactions in vivo” (Oct. 1994)Proc. Natl. Acad. Sci. USABiochemistry 91:10340-10344.
Kitamura et al. “Efficient screening of retroviral cDNA expression libraries” (1995)Proc. Natl. Acad. Sci. USA 92:9146-9150.
Kiyokawa et al., “Mitosis-specific negative regulation of epidermal growth factor receptor, triggered by a decrease in ligand binding and dimerization, can be overcome by overexpression of receptor” (1997)J. Biol. Chem. 272:18656-18665.
Lassar et al., “Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteinsin vivo” (1991)Cell 66:305-315.
Levitzki et al., “Tyrosine kinase inhibition: An approach to drug development” (1995)Science267:1782-1788.
Livneh et al., “Reconstitution of human epidermal growth factor receptors and its deletion mutants in cultured hamster cells” (1986)J. Biol. Chem. 261:12490-12497.
Luo et al., “Mammalian two-hybrid system: A complementary approach to the yeast two-hybrid system” (1997)BioTechniques 22:350-352.
Minden, J.S., “Synthesis of a new substrate for detection oflacZgene expression in liveDrosophilaembryos” (1996)BioTechniques 20:122-129.
Miyawaki et al., “Fluorescent indicator
Blau Helen M.
Mohler William
Rossi Fabio
Bozicevic Field & Francis LLP
Field Bret E.
Lu Frank
Scherer Davis C.
The Board of Trustees of the Leland Stanford Junior University
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