Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-03-14
1991-06-18
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 27, 935 77, 935 78, C12Q 168, C07H 1512, C12N 1500
Patent
active
050249347
ABSTRACT:
The present invention related to a sensitive method of detecting t(14;18) translocations arising from variable breakpoints in the J-region of the immunoglobulin heavy chain locus on chromosome 14. These breakpoints are typical abnormalities of human follicular lymphomas. In particular, the invention utilizes a sequence amplification by polymerase chain reaction in which primers are synthesized which are so designed that one primer will always flank the breakpoint in the J-region regardless of variation in the breakpoint. Consequently, the invention is a highly sensitive tool to detect minimal residual cells carrying the t(14;18) and has potential to identify patients with subclinical disease.
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Fleisher Mindy B.
The Board of Regents The University of Texas System
Wax Robert A.
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