Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1997-12-10
2000-05-30
Weber, Jon P.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435197, 536 187, 548469, C12Q 142, C12N 918, C07H 504, C07D20904
Patent
active
060689889
ABSTRACT:
A method of detecting a phosphatidylinositol-specific phospholipase C enzyme by means of a substrate which is cleaved by said enzyme and yields a dye when the chromophoric portion of the substrate is dimerized and oxidized; the invention teaches using in such method, as a novel substrate, a 3-indoxyl-myo-inositol-1-phosphate compound of formula (I) ##STR1## wherein R is selected from the group consisting of hydrogen and C.sub.1-4 alkyl, while R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are radicals selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, alkyl-substituted amino and sulphonyl; or of a salt of said formula I compound. The invention provides for a safe and sensitive method of detection of potentially pathogenic bacterial activity of such microbes as Bacillus cereus, B. Thuringiensis, Staphy lococcus aureus and various Listeria strains in potentially infected materials including physiological samples or consumable goods such as foods and beverages.
REFERENCES:
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Humm Roland
Schabert Gunter
Spitz Urs P.
Biosynth AG
Hanley Susan
Weber Jon P.
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