Detection of mecA -containing Staphylococcus spp.

Details Detection of mecA -containing Staphylococcus spp. Detection of mecA -containing Staphylococcus spp.

2006-07-11

2006-07-11

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091100, C435S006120, C435S243000, C536S023100, C536S024320, C536S024330

Reexamination Certificate

active

07074599

ABSTRACT:
The invention provides methods to detect mecA-containingStaphylococcusspp. in biological samples using real-time PCR. Primers and probes for the detection ofS. aureusare provided by the invention. Articles of manufacture containing such primers and probes for detecting mecA-containingStaphylococcusspp. are further provided by the invention.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4996143 (1991-02-01), Heller et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5565322 (1996-10-01), Heller
patent: 5683896 (1997-11-01), Hartley et al.
patent: 5702895 (1997-12-01), Matsunaga et al.
patent: 5849489 (1998-12-01), Heller
patent: 5945313 (1999-08-01), Hartley et al.
patent: 5994056 (1999-11-01), Higuchi
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6162603 (2000-12-01), Heller
patent: 269 764 (1988-06-01), None
patent: 0 526 876 (1993-02-01), None
patent: 1 045 033 (2000-10-01), None
patent: 1 160 333 (2001-05-01), None
patent: 1 160 333 (2001-12-01), None
patent: WO 92/05281 (1992-04-01), None
patent: WO 97/46707 (1997-12-01), None
patent: WO 97/46712 (1997-12-01), None
patent: WO 97/46714 (1997-12-01), None
patent: WO 98/48046 (1998-10-01), None
patent: WO 99/19466 (1999-04-01), None
patent: WO 99/45155 (1999-09-01), None
patent: WO 01/12803 (2001-02-01), None
patent: WO 01/23604 (2001-04-01), None
patent: WO 02/18660 (2002-03-01), None
patent: WO 02/61390 (2002-08-01), None
patent: WO 03/068918 (2003-08-01), None
Reischl et al. Rapid identification of Methicillin-resistantStaphylococcus aureusand simultaneous species confirmation using real-time fluorescence PCR. Journal of Clinical Microbiology, vol. 38, No. 6, pp. 2429-2433, Jun. 2000.
Tan et al, Rapid identification of Methicillin-ResistantStaphylococcus auresfrom positive blood cultures by real-time fluorescence PCR. vol. 39, No. 12, p. 4529-4531, Dec. 2001.
Grisold et al. Detection of Methicillin-ResistantStaphylococcus aureusand simultaneous confirmation by automated nucleic acid extraction and real-time PCR. Journal of clinical Microbiology, vol. 40, No. 7, pp. 2392-2397, Jul. 2002.
Uhl et al. Detection of mecA genes inStaphlococcus auresand Coagulase-NegativeStaphylococcusby a rapid PCR method. Abstract C-331, Abstracts of the general meeting of the American Society for Microbiology vol. 100, pp. 208, May 21-25, 2000.
Buck et al. Design Strategies and performance of custom DNA sequencing primers. Biotechniques, vol. 27, No. 3, pp. 528-536, Sep. 1999.
Reischl et al. Journal of Clinical Microbiology, vol. 38, No. 6, pp. 2429-2433, Jun. 2000.
Grisold et al. Journal of Clinical Microbiology, vol. 40, No. 7, pp. 2392-2397, Jul. 2002.
Tan et al. Journal of Clinical Microbiology, vol. 30, No. 12, pp. 4529-4531, Dec. 2001.
GenBank Accession No. AB033763.
Geha et al., “Multiplex PCR for Identification of Methicillin-ResistantStaphylococciin the Clinical Laboratory,”J. Clin. Microbiol., 1994, 32(7):1768-1772.
Ito et al., “Structural Comparison of Three Types ofStaphylococcalCassette ChromosomemecIntegrated in the Chromosome in Methicillin-ResistantStaphylococcus aureus,”Antimicrobial Agents and Chemotherapy, 2001, 45(5):1323-1336.
Bassler et al., “Use of a Fluorogenic Probe in a PCR-based Assay for the Detection ofListeria monocytogenes,” Applied and Environmental Microbiology, 1995, 61(10):3724-3728.
Espy et al., “Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR,”J. Clin. Microbiol., 2000, 38(2):795-799.
Brink et al., “Nucleic Acid Sequence-Based Amplification, A New Method for Analysis of Spliced and Unspliced Epstein-Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR,”J. Clin. Microbiol., 1998, 36(11):3164-3169.
Caplin et al., “LightCycler™ hybridization probes; The most direct way to monitor PCR amplification for quantification and mutation detection,”Biochemica, 1999, 1:5-8.
Espy et al., “Quantification of Epstein-Barr Virus (EBV) Viral Load in Transplant Patients by LightCycler PCR,”Abstracts of the General Meeting of the American Society for Microbiology, 101stGeneral Meeting, May 20-24, 2001, 101:182, Abstract No. C-148.
Espy et al., “Diagnosis of Varicella-Zoster Virus Infections in the Clinical Laboratory by LightCycler PCR,”J. Clin. Microbiol., 2000, 38(9):3187-3189.
Espy et al., “Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR,”J. Clin. Microbiol., 2000, 38(2):795-799.
Espy et al., “Detection of Smallpox Virus DNA by LightCycler PCR,”J. Clin. Microbiol., 2002, 40(6):1985-1988.
Sample et al., “Two Related Epstein-Barr Virus Membrane Proteins are Encoded by Separate Genes,”J. Virol., 1989, 63(2):933-937.
Smith, “Application of Lightcycler Real Time PCR in Clinical Virology,”Clin. Chem. Lab. Med., 2001, Special Supplement, 39:S60, Abstract No. ISW14-2.
Telenti et al., “Detection of Epstein-Barr Virus by Polymerase Chain Reaction,”J. Clin. Microbiol., 1990, 28(10):2187-2190.
Al-Robaiy et al., “Rapid Competitive PCR Using Melting Curve Analysis for DNA Quantification,”BioTechniques, 2001, 31:1382-1388.
Bélanger et al., “Rapid Detection of Shiga Toxin-Producing Bacteria in Feces by Multriplex PCR with Molecular Beacons on the Smart Cycler,”J. Clin. Microbiol., 2002, 40:1436-1440.
Bellin et al., “Rapid Detection of EnterohemorrhagicEscherichia coliby Real-Time PCR with Fluorescent Hybridization Probes,”J. Clin. Microbiol., 2001, 39:370-374.
Chen et al., An Automated Fluorescent PCR Method for Detection of Shiga Toxin-ProducingEscherichia coliin Foods,:Appl. Environ. Microbiol., 1998, 64:4210-4216.
Didenko, “DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications,”BioTechniques, 2001, 31:1106-1121.
Ramotar et al., “Direct Detection of Verotoxin-ProducingEscherichia coliin Stool Samples by PCR,”J. Clin. Microbiol., 1995, 33:519-524.
Livak et al., “Oligonucleotides with Fluorescent Dyes at Opposite Ends Provide A Quenched Probe System Useful for Detecting PCR Product and Nucleic Acid Hybridization,”Genome Research, 1995, 4:357-362.
Ryncarz et al., “Development of a High-Throughput Quantitative Assay for Detecting Herpes Simplex Virus DNA in Clinical Samples,”J. Clin. Microbiol., 1999, 37:1941-1947.
Arthur et al., “Enterococcus faeciumtransposon Tn1546 transposase, resolvase, vanR, vanS, vanH, vanA, vanX, vanY and teicoplanin resistance protein (vanZ) genes, complete cds,” 1993, database accession No. M97297.
Grisold et al., “Detection of Methicillin-ResistantStaphylococcus aureusand Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR,”J. Clin. Microbiol., 2002,40:2392-2397.
Huletsky et al., “Rapid Detection of Vancomycin-Resistant Enterococci Directly from Rectal Swabs by Real-Time PCR Using the Smart Cycler,”Abstracts of the Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Illinois, Sep. 22-25, 2001, 41:409 (Abstract K-1195).
Ito et al., “Staphylococcus aureusDNA, type-I staphylococccal cassette chromosome mec,” 1999, database accession No. AB033763.
“LightCycler-FastStart DNA Master Hybridization Probes,” 1999 Roche Diagnostics GmbH Technical Manual, retrieved from the internet on Feb. 6, 2004: http://www.roche-applied-science.com.
Palladino et al., “Real-time PCR for the rapid detection ofvanAandvanBgenes,”Diagnostic Microbiology and Infectious Disease, 2003, 45:81-84.
Palladino et al., “Rapid Detection ofvanAandvanBGenes Directly from Clinical Specimens and Enrichment Broths by Real-Time Multiplex PCR Assay,”J. Clin. Microbiol., 2003, 41:2483-2486.
Patel et al., “Enterococcus faecali

Affiliated with

Also associated with

Rating

Detection of mecA -containing Staphylococcus spp. does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of mecA -containing Staphylococcus spp., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of mecA -containing Staphylococcus spp. will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3567025