Detection of maize fungal pathogens using the polymerase chain r

Details Detection of maize fungal pathogens using the polymerase chain r Detection of maize fungal pathogens using the polymerase chain r

1996-11-01

1998-09-01

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 913, 536 231, 536 241, 536 242, 536 243, 536 2433, 935 6, 935 17, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

058009973

ABSTRACT:
Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5324632 (1994-06-01), Weisburg et al.
patent: 5447848 (1995-09-01), Barns
patent: 5585238 (1996-12-01), Ligon et al.
Olivier et al. Phytopathology vol. 86 No. 11, Supplement pp. S1 and S12, Nov. 1996.
Bentolila et. al. Theoret. Appl. Genet 82:393-8 (Sep. 1991).
Olivier et. al. Phytopathology 86(11):812 (Nov. 1996).
Johanson et al. Use of PCR for detection of Mycosphaerella fijiensis and M. musicola", the causal agents of Sigatoka leaf spots in banana and plantain", Mycol. Res., 97:670-674 (1993).
Nazar, R.N., et al, "Potential use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of verticillium wilt pathogens", Physiol. and Molec. Plant Pathol., 39:1-11 (1991).
Poupard et al., "Molecular characterization of Pseudocercosporella herpotrichoides isolates by amplification of ribosomal DNA internal transcribed spaces", Plant Pathology, 42: 873-881 (1993).
Schesser et al., "Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants", Applied and Environ. Microbiol., 57(2):553-556 (1991).
Stratagene Catalog, 1988, p. 39.
Tisserat et al., "Selective Amplification of rDNA Internal Transcribed Spacer Regions to Detect Ophiosphaerella korrae and O. herpotricha", Phytopathology, 84(5): 478-482 (1994).
White, T.J., et al., "Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics", In: PCR Protocols; Academic Press Inc., pp. 315-322 (1990).
GenBank Accession No. L08734, computer printout, Feb. 5, 1993.
Xue et al., "Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences", Physiological and Molecular Plant Pathology, 41: 179-188 (1992).

Affiliated with

Also associated with

Rating

Detection of maize fungal pathogens using the polymerase chain r does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of maize fungal pathogens using the polymerase chain r, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of maize fungal pathogens using the polymerase chain r will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-268989