Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-10-24
2011-11-29
Kim, Young J (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S287200
Reexamination Certificate
active
08067169
ABSTRACT:
Method and apparatus which uses harmonic cantilevers, such as used in atomic force microscopy, to detect variations in the attractive and repulsive forces on a solid surface as a result of macromolecular binding, for example, hybridization of a single stranded DNA molecule attached to the surface with another DNA molecule. The complexed macromolecule is less flexible than an uncomplexed molecule. It will typically have more negative charge due to amino acids or DNA monomers. Both stiffness of the surface and the attractive capillary forces will change after binding and may be detected. The present methods and materials enable ultraflat surfaces for the macromolecule deposition, and may include the use of a gold-coated mica substrate and a self-assembling monolayer.
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Persson Henrik
Quate Calvin F.
Sahin Ozgur
Solgaard Olav
Aston David J.
Bertagna Angela M
Kim Young J
Peters Verny LLP
The Board of Trustees of the Leland Stanford Junior University
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