Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-11-05
2000-12-05
Arthus, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
061565083
ABSTRACT:
The present invention provides primers which can be used for M. tuberculosis complex-specific detection of .alpha.-antigen DNA in a diagnostic assay performed on clinical specimens or in a culture-confirmation assay following growth of the organism in vitro. These primers and probes can also be employed in a reverse transcriptase-mediated amplification system for M. tuberculosis complex .alpha.-antigen mRNA. Such an assay provides a means by which to determine the viability of M. tuberculosis complex organisms either in clinical specimens or when grown in culture. The specific DNA or mRNA target region can be amplified using SDA, PCR, LCR, Nucleic Acid Sequence Based Amplification (NASBA), Self-sustained Sequence Replication (3SR) or Q.beta. Replicase-mediated systems. Also described are methods for the detection of the products of amplification with a radiolabeled probe by chemiluminescent assay or fluorescence polarization analysis.
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Cave Mac Donald
DesJardin Lucy Ellen
Eisenach Kathleen Davis
Hellyer Tobin James
Spears Patricia Anne
Adler Benjamin Aaron
Arthus Lisa B.
Sonaya Jehanne
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