Detection of loss of the wild-type P53 gene and kits therefor

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 691, 435810, 436501, 436 63, 514 44, 536 231, 536 241, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168, C07H 2104

Patent

active

055276768

ABSTRACT:
Methods and kits are provided for assessing mutations and/or loss of the p53 gene in human tumors. Both deletion mutations and point mutations in p53 are observed in the same human tumor cells and these mutations are clonal within the cells of the tumor. Loss of wild-type p53 genes is responsible for neoplastic progression.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Lamb et al. (1986), Mol. Cell. Biol., vol. 6, No. 5, pp. 1379-1385.
Zakut-Houri et al. (1985), EMBO J., vol. 4, No. 5, pp. 1251-1255.
Harris et al. (1986) Mol. Cell. Biol., vol. 6, No. 12, pp. 4650-4656.
Matlashewski et al. (1987) Mol. Cell. Biol., vol. 7, No. 2, pp. 961-963.
Buchman et al. (1988), Gene, vol. 70, pp. 245-252.
Green, "When the Products of Oncogenes and Anti-Oncogenes Meet," Cell (1989), 56:1-3.
DeCaprio et al., "SV40 Large Tumor Antigen Forms a Specific Complex with the Product of the Retinobastoma Susceptibility Gene," Cell (1988), 54:275-283.
Hinds et al., "Mutation is Required to Activate the p53 GEne for Cooperation with the ras Oncogene and Transformation," J. Virol., (1989), 63:739-746.
Finlay et al., "Activating Mutations for Transformation by p53 Produce a Gene Product that Forms an hsc70-p53 Complex with an Altered Half-Life," Molecular and Cellular Biology (1988), 8:531-539.
Mercer et al., "Microinjection of Monoclonal Antibody to Protein p53 Inhibits Serum-Induced DNA Synthesis in 3T3 Cells," Proc. Natl. Acad. Sci. USA (1982), 79:6309-6312.
Hicks et al., "Integration of Friend Murine Leukemia Virus into Both Alleles of the p53 Oncogene in an Erythroleukemic Cell Line," J. Virol. (1988), 62:4752-4755.
Milner et al., "SV40-53K Antigen: A Possible Role for 53K in Normal Cells," Virology (1981), 112:785-788.
Sturzbecher et al., "Characterization of Mutant p53-hsp72/73 Protein-Protein Complexes by Transient Expression in Monkey COS Cells," Molecular and Cellular Biology (1988), 8:3740-3747.
Eliyahu et al., "Meth A Fibrosarcoma Cells Express Two Transforming Mutant p53 Species," Oncogene (1988), 3:313-321.
Rovinski et al., "Immortalization of Rat Embryo Fibroblasts by the Cellular p53 Oncogene," Oncogene (1988), 2:445-452.
Eliyahu et al., "Participation of p53 Cellular Tumour Antigen in Transformation of Normal Embryonic Cells," Nature (1984), 312:646-649.
Parada et al., "Cooperation Between Gene Encoding p53 Tumour Antigen and Ras in Cellular Transformation," Nature (1984) 312:649-651.
DeLeo et al., "Detection of a Transformation-Related Antigen in Chemically Induced Sarcomas and Other Transformed Cells of the Mouse," Proc. Natl. Acad. Sci. USA, (1979), 76:2420-2424.
Reich et al., "Growth Regulation of a Cellular Tumour Antigen, p53, in Non-transformed Cells," Nature (1984), 308:199-201.
Jenkins et al., "Cellular Immortalization by a cDNA Clone Encoding the Transformation-Associated Phosphoprotein p53," Nature (1984), 312:651-654.
Koshland, "p53 Sweeps Through Cancer Research", Science, 262:1958-1959 (1993).
DeCaprio, et al., "SV40 Large Tumor Antigen Forms a Specific Complex With the Product of the Retinoblastoma Susceptibility Gene", Cell 54:275-283 (1988).
Harlow, et al., "Association of Adenovirus Early-Region 1A Proteins With Cellular Polypeptides", Molecular and Cellular Biology, 6(5):1579-1589 (1986).
Harlow, et al., "Monoclonal Antibodies Specific for Simian Virus 40 Tumor Antigens", Journal of Virology, 39(3):861-869 (1981).
Gannon, et al., "p53 and DNA Polymerase .alpha. Complete for Binding to SV40 T Antigen", Nature, 329:456-458 (1987).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Detection of loss of the wild-type P53 gene and kits therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of loss of the wild-type P53 gene and kits therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of loss of the wild-type P53 gene and kits therefor will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-222125

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.