Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1997-06-20
2000-03-07
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
73 179, 73794, 73795, 73826, 73833, G01N 3348
Patent
active
060339136
ABSTRACT:
A method and apparatus for measuring ligand binding to DNA or protein uses changes in the mechanical properties of strips of the DNA or protein to detect the formation of ligand complexes. Samples strips are prepared by pouring protein solution onto a glass surface, drying the solution, and causing the protein to be cross-linked; the cross-linked film is scraped off, cut into strips, and mounted in the stress/strain measuring apparatus, which includes force and strain transducers. The sample is held by arms and can be immersed into solutions. The arms are attached to transducers. The apparatus includes a sample holder. The sample films may include end reinforcements and may be pinned or glued to the arms.
REFERENCES:
patent: 4114420 (1978-09-01), Browning
patent: 5510481 (1996-04-01), Bednarski et al.
Berry, B.S. et al., "Bending-cantilever method for the study of moisture swelling in polymers.", IBM Journal of Research and Development, vol. 28, No. 6, pp. 662-667 (1984).
Morozov, Victor et al., "Mechanical detection of interaction of small specific ligands with proteins and DNA in cross-linked samples.", Analytical Biochemistry, vol. 201, pp. 68-79 (1992).
Morozov Victor
Morozova Tamara
Alexander Lyle A.
New York University
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