Detection of ligand-antiligand complex formation by...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S007210, C435S004000, C435S287200, C435S288700, C436S501000, C436S518000, C436S524000, C436S527000, C436S528000, C436S535000, C422S068100, C422S081000, C422S091000

Reexamination Certificate

active

07011948

ABSTRACT:
Disclosed are methods for analyzing molecular binding events in which the formation of ligand/antiligand complexes can be directly detected in a mixture without requiring separation of the components of the mixture from each other by measuring bulk properties of the mixture (i.e., properties that have contributions from several or all of the components present in the mixture). Using these techniques, it is possible to screen libraries without labeling either the target antiligand or ligand. The invention also provides a method for determining the strength of ligand/antiligand binding by further analysis of the same signals.

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patent: 5656428 (1997-08-01), McAllister et al.
patent: 6048692 (2000-04-01), Maracas et al.
patent: 0519250 (1992-12-01), None
Lieb, Simplex Method of Nonlinear Least Squares- A Logical Complementary Method to Linear Least Squares Analysis of Dat Aug. 1997, Journal of Chemical Education, vol. 74, No. 8, p. 1008.
Malmqvist et al., “Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins” 1997, Curent Opinion in Chemical Biology, 1:378-383.
McKee et al., “Real-time chemical sensing of aqueous ethanol glucose mixtures”, IEEE Transactions on Instrumentation and Measurement, 49(1):114-119 (2000).
Stuchly et al., “Coaxial line reflection methods for measuring dielectric properties of biological substances at radio and microwave frequencies—A review”, IEEE Transactions on Instrumentation and Measurement, vol. IM-29, No. 3, Sep. 1980, pp. 176-183.

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