Detection of influenza a virus by polymerase chain reaction (PCR

Details Detection of influenza a virus by polymerase chain reaction (PCR Detection of influenza a virus by polymerase chain reaction (PCR

1990-08-31

1993-02-16

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 75, 435 29, 435 91, 536 2372, 935 2, 935 16, C12Q 170, C12Q 168, C12Q 102, C07H 1512

Patent

active

051870606

ABSTRACT:
It was possible to increase substantially the sensitivity of detection of influenza A virus by carrying out a polymerase chain reaction (PCR), using oligonucleotide sequences (amplimers) from the relatively conserved region of the viral hemagglutinin gene, preceded by reverse transcription (RT). The reverse transcription and the PCR are insensitive to single mutations by base exchange in this case, as long as the 3' end of the amplimer is not affected.

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