Detection of hydrolyzing enzymes

Details Detection of hydrolyzing enzymes Detection of hydrolyzing enzymes

1986-01-17

1988-11-01

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 18, 436169, 436170, 436807, 436810, C12Q 140, C12Q 134, C12Q 100, G01N 2177

Patent

active

047820187

ABSTRACT:
A composition of a polysaccharide with a detectable material appended thereto and a 1,3-dione having a methylene group between the two carbonyls of the 1,3-dione, which methylene group has at least one ionizable hydrogen, provides accurate results in assays of hydrolyzing enzymes such as amylase. The composition can be incorporated in the reagent zone of a dry test element having a reagent zone and a registration zone.

REFERENCES:
patent: 3694318 (1972-09-01), Klein et al.
patent: 4144306 (1979-03-01), Figueras
patent: 4478944 (1984-10-01), Gross et al.
patent: 4544631 (1985-10-01), Rauscher et al.
Spayd, Richard W. et al., "Multilayer Film Elements for Clinical Analysis: Applications to Representative Chemical Determinations", Clin. Chem., 24(8), 1343-1350, 1978.

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