Detection of hydrolyzing enzymes

Details Detection of hydrolyzing enzymes Detection of hydrolyzing enzymes

1974-02-08

1978-01-03

Naff, David M.

Textiles: fiber preparation

195 99, G01N 3114

Patent

active

040665097

ABSTRACT:
The activity, or concentration, of a hydrolyzing enzyme, e.g., an endo-enzyme, is determined by providing a detection layer comprising a carrier matrix and a water-insoluble substrate dispersed in said carrier matrix which substrate is detectably marked, e.g., dyed, and which substrate, upon action of a hydrolyzing enzyme thereon, is broken down into soluble fragments capable of diffusing through the carrier matrix to cause formation of an unmarked area in said detection layer; and measuring the size of the unmarked area as a measure of the activity of concentration of said enzyme.

REFERENCES:
patent: 3416998 (1968-12-01), Streitfeld
patent: 3676303 (1972-07-01), Ingelman et al.
Sbarra, et al., A Plate Assay for Elastase Nature, vol. 188, 1960 (pp. 322-323).
Hall; D. A., The Identification and Estimation of Elastase in Serum and Plasma, Biochem. J., vol. 101, 1966, (pp. 29-36).
Merkel; J. R., Method for Detecting and Isolating Proteolytic Marine Bacteria, Journal of Bacteriology, vol. 89, No. 3, 1965 (pp. 903-904).
Lanyi; et al., Fluorescent Method for The Detection of Excreted Ribonuclease Around Bacterial Colonies Journal of Bacteriology, vol. 92, No. 5, 1966 (pp. 1469-1472).

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