Detection of human papillomavirus mRNA in cervical smears

Details Detection of human papillomavirus mRNA in cervical smears Detection of human papillomavirus mRNA in cervical smears

1995-06-07

1998-05-12

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 912, 935 77, 935 78, C12Q 170, C12Q 168, C12P 1934

Patent

active

057503346

ABSTRACT:
The invention relates to the direct detection of human papillomavirus MRNA and DNA. The recently developed polymerase chain reaction was modified in order to improve the sensitivity and specificity. By choosing appropriate oligonucleotide primers (amplimers) and reaction temperatures, individual HPV genes from among the total cell DNA can be identified and amplified to an extent such that non-isotopic detection is possible. In addition, use of reverse transcription permits selective amplification of spliced mRNA and thus permits indication of premalignant or malignant conditions and/or lesions.

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