Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-06-10
1996-03-12
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 4, 435810, 436 63, 436 74, C12Q 104, C12Q 102, G01N 3348, G01N 3320
Patent
active
054985285
ABSTRACT:
A method for detecting Helicobacter pylori is disclosed which method involves contacting a sample suspected of containing Helicobacter pylori with a medium which provides for substantially selective growth of Helicobacter pylori, incubating the sample with the medium for a time sufficient for detection of Helicobacter pylori growth and detecting the growth and thereby reducing the presence of Helicobacter pylori within the sample. The methodology employs a wide range of a different culture mediums which are modified specifically for the selective growth and specific detection of Helicobacter pylori. A typical medium includes Columbia broth supplemented with urea and a pH indicator. The methodology provides for a relatively high degree of sensitivity (i.e., small numbers of bacteria present within a sample are detected) as well high selectivity (i.e., the method provides for a low percentage of false positives). Various kits used in connection with the method are designed so that they can be used by unskilled users in an "at home" setting. The kits and methodology are economical, easily used and provide highly accurate results within a relatively short period of time (e.g., 3 days or less).
REFERENCES:
patent: 4101382 (1978-07-01), Chang
patent: 4282316 (1981-08-01), Modrovich
patent: 4748113 (1988-05-01), Marshall
patent: 4923801 (1990-05-01), Marshall et al.
patent: 4947861 (1990-08-01), Hamilton
patent: 5200344 (1993-04-01), Blaser et al.
patent: 5262156 (1993-11-01), Alemohammad
patent: 5286492 (1994-02-01), Dettmar et al.
Cellini et al, J. Clin Micro., vol. 30, pp. 1351-1353, May 1992.
Tee et al, J. Clin Micro., vol. 29, No. 11, pp. 2587-2589, (1991).
Cellini et al., "New Plate Medium for Growth and Detection of Urease Activity of Helicobacter pylori," J. Clin. Microbiol. (1992) 30:1351-1353.
Endtz et al., "Comparison of Six Media, Including a Semisolid Agar, for the Isolation of Various Campylobacter Species from Stool Specimens," J. Clin. Microbiol. (1991) 29:1007-1010.
Ferguson et al., "Isolation of Helicobacter pylori from Saliva," J. Clin. Microbiol. (1993) 31:2802-2804.
McNulty and Wise, letter, "Rapid Diagnosis of Campylobacter-Associated Gastritis," Lancet (1985) pp. 1443-1444.
Schrader et al., "A Role for Culture in Diagnosis of Helicobacter pylori-Related Gastric Disease," Am. J. Gastroenterology (1993) 88:1729-1733.
Secker et al., "Gas-Permeable Lifecell Tissue Culture Flasks Give Improved Growth of Helicobacter pylori in a Liquid Medium," J. Clin. Microbiol. (1991) 29:1060-1061.
Shahamat et al., "Evaluation of Liquid Media for Growth of Helicobacter pylori," J. Clin. Microbiol. (1991) 29:2835-2837.
Tee et al., "Comparative Evaluation of Three Selective Media and a Nonselective Medium for the Culture of Helicobacter pylori from Gastric Biopsies," J. Clin. Microbiol. (1991) 29:2587-2589.
Xia et al., "Enhanced Cultivation of Helicobacter pylori in Liquid Media," J. Clin. Pathol. (1993) 46:750-753.
Bozicevic Karl
Francis Carol L.
Leary Louise N.
Wityshyn Michael G.
LandOfFree
Detection of helicobacter pylori does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection of helicobacter pylori, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of helicobacter pylori will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2099398