Detection of fungal pathogens using the polymerase chain reactio

Details Detection of fungal pathogens using the polymerase chain reactio Detection of fungal pathogens using the polymerase chain reactio

1994-04-25

1996-12-17

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 915, 536 231, 536 242, 536 2432, 536 2433, 935 6, 935 8, 935 9, 935 17, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

055852386

ABSTRACT:
DNA sequences from the Internal Transcribed Spacer of the ribosomal RNA gene region are described for different species and strains of Septoria, Pseudocercosporella and Mycosphaerella. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5324632 (1994-06-01), Weisburg et al.
patent: 5447848 (1995-09-01), Barns et al.
Tisserat et al., "Selective Amplification of rDNA Internal Transcribed Spacer Regions to Detect Ophiosphaerella korrae and O. herpotricha", Phytopathology, 84(5): 478-482 (1994).
Xue et al., "Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primrers from ribosomal internal transribed spacer sequences", Physiological and Molecular Plant Pathology, 41: 179-188 (1992).
Stratogene Catalog, 1988, p. 39.
GenBank Accession No. U04237, computer printout, Jan. 4, 1994.
Johanson A., and Jeger, M. J., "Use of PCR for Detection of Mycosphaerella fijiensis and M. musicola, the Causal Agents of Sigatoka Leaf Spots in Banana and Plantain", Mycol. Res., 97:670-674 (1993).
Nazar, R. N., et al, "Potential Use of PCR-amplified Ribosomal Intergenic Sequences in the Detection and Differentiation of Verticillium Wilt Pathogens", Physiol. and Molec. Plant Pathol., 39:1-11 (1991).
Schlesser, K., et al., "Use of Polymerase Chain Reaction to Detect the Take-All fungus, Gaeumannomyces graminis, in Infected Wheat Plants", Applied and Environ. Microbiol., 57(2):553-556 (1991).
White, T. J., et al., "Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics", In: PCR Protocols; Eds.: Innes et al., al., 315-322 (1990).
Poupard, P. et al., "Molecular Characterization of Pseudocercosporella herpotrichoides Isolates by Amplification of Ribosomal DNA Internal Transcribed Spacers", Plant Pathology, 42:873-881 (1993).

Affiliated with

Also associated with

Rating

Detection of fungal pathogens using the polymerase chain reactio does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of fungal pathogens using the polymerase chain reactio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of fungal pathogens using the polymerase chain reactio will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1990366