Detection of fibrin monomer and composition therefor

Details Detection of fibrin monomer and composition therefor Detection of fibrin monomer and composition therefor

1978-11-13

1980-07-01

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 3316, G01N 3102, G01N 2122

Patent

active

042104203

ABSTRACT:
A quantitative method and composition for precipitating fibrin monomers from plasma in the presence of fibrinogen, with only a small amount, if any, of fibrinogen being precipitated. A buffered precipitating agent is provided having a pH in the range of 6.5.+-.0.5, the buffer being one having a pKa in the range of 6 to 7 and protamine sulfate at a level of 0.025 to 0.2% by weight. Suitable buffers are 2-(N-morpholino)ethanesulfonic acid, sodium phosphate, histidine, maleic acid and imidazole. Analysis of the amount of fibrin present can be made by analyzing for protein using preferably a basic urea solution containing sodium hydroxide and measuring the absorbance at 282 nanometers.

REFERENCES:
patent: 3915640 (1975-10-01), Turner
patent: 4090846 (1978-05-01), Buck
C. A. Vega et al., Anal. Chem., 48, (9), 1293-1296, (Aug. 1976).

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