Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1989-12-08
1991-10-22
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, C12Q 102
Patent
active
050595270
ABSTRACT:
A method and apparatus for inferring and quantifying the presence and identifying endotoxins of Gram-negative bacteria in a bacteria-containing sample comprising obtaining a sample, extracting any lipids from the sample using supercritical CO.sub.2, hydrolyzing the lipid insoluble residue with a mild acid catalyst, derivatizing the hydroxy fatty acids, and analyzing the sample using a gas chromatograph and mass spectrometer. The results of the gas chromatograph and mass spectrometer analysis allow detection of as few as 10 bacteria or parts of bacteria in a 100 ml sample and specification of the type of Gram-negative bacterial group.
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Mittelman Marc W.
White David C.
Mann Michael A.
Rosen Sam
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