Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1996-07-12
2000-01-18
Housel, James C.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
4242831, 436 13, 436829, 554 79, A61K 950, G01N 3100, C07F 902
Patent
active
06015716&
ABSTRACT:
Endotoxin incorporated into liposomes, lipid bilayers or lipid complexes can be detected by combining an aqueous suspension of the liposomes, lipid complexes or lipid bilayers with a suitable detergent. Preferable detergents, e.g., Lubrol-PX.TM. or a polyoxyethylene ether, solubilize the lipid bilayers, liposomes or lipid complexes at acceptable lipid concentrations, forming micelles therefrom which contain lipid bilayer, liposome or lipid complex lipid, detergent and endotoxin, should it be present. The micelles are then assayed for the presence of endotoxin.
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Cabral-Lilly Donna J.
Franklin J. Craig
Harmon Paul A.
Janoff Andrew S.
Reed Robert A.
Goodman Rosanne
Housel James C.
Rubin Kenneth B.
Swartz Rodney P.
The Liposome Company Inc.
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