Detection of DNA/RNA by fluorescence polarization

Details Detection of DNA/RNA by fluorescence polarization Detection of DNA/RNA by fluorescence polarization

1994-05-23

1999-12-28

Priebe, Scott D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 243, C12Q 168, C07H 2100, C12P 1934

Patent

active

060079843

ABSTRACT:
A homogeneous method for detecting amplified RNA or DNA target sequences utilizes signal-labelled DNA or RNA probes which show detectably increased fluorescence polarization when hybridized to target sequences. A convenient one-step analytical procedure requiring no nucleic acid extraction or signal separation step is thereby provided.

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