Detection of dioxin-like compounds by detection of transformed A

Details Detection of dioxin-like compounds by detection of transformed A Detection of dioxin-like compounds by detection of transformed A

1996-02-14

2000-10-03

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 436503, 436519, 436530, 436815, G01N 33548, G01N 33567

Patent

active

061271365

ABSTRACT:
A method of detecting dioxin-like compounds consisting essentially of the group of compounds: polychlorinated dibenzodioxins, polychlorinated dibenzofurans, polychlorinated biphenyls, and structural analogues thereof which exhibit biological activity characteristic of polychlorinated dibenzodioxins, polychlorinated dibenzofurans, polychlorinated biphenyls. The assay includes: 1) an inactive Ah receptor in a form capable of binding to the dioxin-like compounds and being transformed to an active form that forms a complex with ARNT and binds a dioxin responsive element, and 2) a quantity of ARNT sufficient to optimize Ah receptor transformation. A test sample is contacted with the assay under conditions effective to bind the dioxin-like compounds to the Ah receptor and allow transformation of the Ah receptor to an active form that forms a complex with ARNT. The presence of the complex containing the transformed Ah receptor and the ARNT is detected. The presence of the complex containing the transformed Ah receptor and the ARNT is detected with an antibody with a region capable of binding to the ARNT when associated with the complex.

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