Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1997-12-02
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 231, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
056934726
ABSTRACT:
A method and kit for the detection of Cryptosporidium parvum in aquatic and biological samples such as surface water or feces. The method relies on the use of primers to detect all or a portion of at least one DNA sequence characteristic of C. parvum, the sequence being all or part of the genomic regions referred to as 38G and HemA contained within recombinant plasmids pINV38G, and pHem4, respectively.
REFERENCES:
Knapp et al., "Protection Of Aotus Monkeys From Malaria Infection By Immunization With Recombinant Hybrid Proteins", Infection and Immunity, Jun. 1992, pp. 2397-2401.
Webster et al., "Detection of Cryptosporidium parvum Using A Specific Polymerase Chain Reaction", Veterinary Parasitology, 50 (1993) 35-44.
M. Steele; T. Kuhls; D. Mosier; K. Nida; W. Elliott; "Identification of a Cryptosporidium parvum Genomic Region that Encodes a Protein Which Manifests Hemolytic Activity"; University of Oklahoma Health Sciences Center; Oklahoma City, OK, USA; 94th General Meeting of the American Society for Microbiology, Las Vegas, Nevada, USA, May 23-27, 1994. Abstracts of the General Meeting of the American Society for Microbiology 94; 1994.
J. Protozool., vol. 38, No. 6, Nov. 1991, pp. 74s-76s, XP000615257, Kuhls et al.: "Effects of Carbohydrates and Lectins on Cryptosporidial Sporozoite Penetration of Cultured Cell Monolayers".
American Journal of Tropical Medicine & Hygiene, vol. 45, No. 6, 1991, pp. 688-694, XP000405969, Laxer et al.: "DNA Sequences for the Specific Detection of Cryptosporidium Parvum by the Polymerase Chain Reaction".
Dykstra et al. J. of Protozool. 38: 76S-78S, 1991.
Kuhls Thomas L.
Nida S. Kay
Steele Marilyn I.
Jones W. Gary
Rees Dianne
The Board of Regents of the University of Oklahoma
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