Detection of chemical mutagens in marker rescue assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 6, 435 29, 4351721, C12Q 170, C12Q 168, C12Q 102, C12N 1500

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044697864

ABSTRACT:
The present application discloses a short-term microbial assay useful in detecting chemical mutagens. A Bacillus subtilis which is infected with a strain of the temperate bacteriophage .phi.105 having a conditional lethal mutation is specifically illustrated. In the assay exogenous DNA from wild-type .phi.105 is reacted with a potential mutagen and then transfected into the B. subtilis carrying a mutated .phi.105 DNA. After plating, an increase in the frequency of clear plaques signifies that the test compound is mutagenic.
The present invention provides a short-term mutagenicity test which detects frameshift as well as base substitution mutations induced by chemicals.

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