Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1984-08-23
1988-08-23
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 34, G01N 3353, C12Q 104
Patent
active
047660651
ABSTRACT:
The invention comprises a method for detecting cell proteins of microorganisms, such as the principal outer membrane protein of Chlamydia trachomatis having a mean molecular weight of 39,500 daltons. The method includes the steps of adding a buffer salt solution to a specimen suspected of containing bacterial antigens, raising the pH of the buffered solution so produced, incubating the solution, adding a neutralizing buffer to the solution to lower the pH, and assaying the sample by conventional immunoassay techniques. Optionally the sample solution is heated prior to incubation and then cooled afterwards before adding the neutralizing buffer.
REFERENCES:
patent: 3855197 (1974-12-01), Hirsch et al.
patent: 3891508 (1975-06-01), Merrick
patent: 4118469 (1978-10-01), Caldwell et al.
patent: 4427782 (1984-01-01), Caldwell et al.
patent: 4497899 (1985-02-01), Armstrong et al.
Mosier Larry
Petersen John
Becton Dickinson and Company
Deck Randall E.
McBride James R.
Warden Robert J.
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