Detection of candida albicans

Details Detection of candida albicans Detection of candida albicans

1994-03-07

1996-08-13

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 72, 435 911, 435 912, 536 243, 536 2432, 536 2433, C07H 2102, C07H 2104, C12P 1534, C12Q 168

Patent

active

055455255

ABSTRACT:
The present invention relates to a DNA probe adapted to be used as a PCR target for the detection of the presence of C. stellatoidea and a subgroup of C. albicans strains in a biological sample, which comprises the nucleic acid sequence set forth in SEQ ID NO:1 or any functional analogs thereof wherein the hybridization of the probe and the rRNA of C. albicans is substantially preserved. The present invention also relates to a method for the detection of trace amount of C. stellatoidea or a subgroup of C. albicans in a biological sample, which comprises the steps of: a) isolating DNA from said biological sample; b) amplifying said isolated DNA of step a) with paired oligonucleotides: 5' primer: 5' AAC TTA GAA CTG GTA CGG 3' (SEQ ID NO:2), 3' primer: 5' AGT AGA TAG GGA CAG TGG 3' (SEQ ID No:3); or 5' GAC TCT CAA CCT ATA AGG 3' (SEQ ID NO:4), 3' primer: 5' TTA AGC ATT GCT CCA AGA 3' (SEQ ID NO:5); c) isolating amplified DNA of step b) and determining the presence or absence of C. stellatoidea or a subgroup of C. albicans in said biological sample by direct examination of the amplified products on stained gel or by hybridization of the amplified product with the DNA segment used as target.

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